Medical Evidence
Effects of glucosamine hydrochloride
and chondroitin sulphate, alone and in combination, on normal
and interleukin-1 conditioned equine articular cartilage explant
metabolism Dechant JE, Baxter GM,
Frisbie DD, Trotter GW, McIlwraith CW. Equine Orthopaedic Research
Laboratory, Department of Clinical Sciences, College of Veterinary
Medicine and Biomedical Sciences, Colorado State University, Fort
Collins, Colorado 80523, USA.
Background: Clinical trials
in human and veterinary literature have documented the benefits
of oral nutraceutical joint supplements containing glucosamine
(GU) and chondroitin sulphate (CS) to treat mild to moderate osteoarthritis,
but the effects of these components have not yet been conclusively
determined. OBJECTIVES: To assess varying dosages of GU and CS
on normal and interleukin-1alpha (IL-1) conditioned equine cartilage
explants and rationalise the use of these products. HYPOTHESIS:
Treatment would not be detrimental to cartilage metabolism and
higher dosages and the combination of GU and CS would be more
beneficial than lower dosages and. GU or CS alone. METHODS: Articular
cartilage explants collected from the femoral trochlea and condyles
were cultured in normal and IL-1 conditioned media. Treatment
groups included 0, 12.5, 25,125 and 250 microg/ml concentrations
of GU alone, CS alone, or GU+CS in combination. Glycosaminoglycan
(GAG) synthesis and total GAG content in the explants and media
were analysed. RESULTS: There were no detrimental effects of GU,
CS or GU+CS on cartilage metabolism. High dosages of GU+CS reduced
total GAG release into the media (degradation). CONCLUSIONS: Our
results suggests that GU+CS may prevent cartilage GAG degradation.
POTENTIAL RELEVANCE: The combination of GU and CS may be more
effective in preventing or treating osteoarthritis in horses than
either product alone.
Source: Equine Vet J. 2005
May;37(3):227-31.
Dr. Theo's Comments: Another
study to show the benefit of the combination of the supplements
versus either alone. Supports Dr. Theo's original premise in The
Arthritis Cure.