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Beneficial Effect Of Cartilage Disease-Modifying Agents Tested In Chondrocyte Cultures And A Rabbit Instability Model Of Osteoarthrosis. L Lippiello, J Woodward, R Karpman, T A Hammad. Phoenix, AZ, Edgewood, Md.

Summary: Dietary supplements of disease-modifying agents were tested separately and in combination for their ability to retard progression of cartilage lesions in a rabbit instability model of osteoarthritis. Surgical instability (Hulth) was induced in 2-3 Kg NZW rabbits. Post-operatively, animals were exercised for 1 hour 3 times per week. Group 1 controls (12) were fed standard Teklad diet; group 2 (12), 3 (6) and 4 (6) had diets supplemented with 2% by weight Cosamin DS*, and comparable levels of chondroitin sulfate or glucosamine HCl respectively. Animals were sacrificed at 16 weeks and the weight-bearing portion of the medial condyles evaluated quantitatively with a modified Mankin grading system using Safranin-O stained slides. The extent and severity of lesions was measured as mm linear involvement for each grade of severity using Image Pro software and a Polaroid Slide Scanner. Lesions were grouped as mild (1-3), moderate (4-7) or severe (>8). Statistical analysis utilized Student t-test and Wilcoxon rank sum test.

Results: Cosamin? DS fed animals had no severe lesions and a significant substantial reduction in the extent of moderate lesions compared to controls (p<0.003). Chondroitin sulfate and glucosamine-fed animals had less moderate and severe tissue involvement than controls but not to the extent of the Cosamin? DS group. No metabolic changes were noted in noninvolved normal humeral cartilage nor was there any apparent organ pathology upon necropsy. In vitro studies with isolated chondrocytes confirmed a synergistic effect of glucosamine HCL + chondroitin sulfate on stimulation of proteoglycan synthesis (p<0.04). The data suggests that the disease-modifying effect of a mixture of glucosamine HCL and chondroitin sulfate was synergistic and superior than either agent alone insofar as stimulating proteoglycan synthesis and retarding the development of cartilage lesions. *A combination of glucosamine HCl and low molecular weight chondroitin sulfate. Nutramax Laboratories, Edgewood, MD.

Disclosure: Study supported by Nutramax Laboratories, Edgewood, Maryland

Source: American College of Rheumatology 1999 Annual Meeting, Boston, MA

Presentation Date: Monday, November 15, 1999


 
 

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